EXTRACTMAN®: Protein G Magnetic Bead Comparisons for Immunoprecipitation
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چکیده
Immunoprecipitation (IP) and Co-IP are common techniques for isolating proteins and identifying protein-protein interactions, respectively. Exclusion-based sample preparation (ESPTM) with EXTRACTMAN® (Figure 1) allows for rapid, yet gentle washing of protein solutions during these procedures. EXTRACTMAN has been shown to be ideal for isolating elusive, weakly-bound protein complexes by Co-IP using Dynabeads® and for immunoprecipitation of RNA binding proteins.1,2 This application note describes a comparison of superparamagnetic particles (magnetic beads) conjugated to Protein G for immunoprecipitation using EXTRACTMAN.
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تاریخ انتشار 2017